Human Matrix metalloproteinase-16 (MMP16) ELISA Kit

SKU: AE58329HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P51512

Abbreviation: MMP16

Alternative Names: C8orf57; DKFZp761D112; MMP-X2; MT-MMP2; MT-MMP3; MT3-MMP; matrix metalloproteinase 16|membrane-type matrix metalloproteinase 3|membrane-type-3 matrix metalloproteinase

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.064 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate MMP16 in samples. An antibody specific for MMP16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene produces at least two transcripts, one which encodes a membrane-bound form and another a soluble form of the protein. Both forms of the protein activate MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).