Human Lim Domain Kinase 1, LIMK1 ELISA Kit

SKU: E5581Hu

Size:96 Tests-1 Kit

Background:Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways (PubMed: 10436159, PubMed: 11832213, PubMed: 12807904, PubMed: 15660133, PubMed: 16230460, PubMed: 18028908, PubMed: 22328514, PubMed: 23633677). Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop (PubMed: 10436159). LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton (PubMed: 11832213, PubMed: 15660133, PubMed: 16230460, PubMed: 23633677). In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation (PubMed: 11832213, PubMed: 15660133, PubMed: 16230460, PubMed: 23633677). Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly (PubMed: 18028908). Stimulates axonal outgrowth and may be involved in brain development (PubMed: 18028908). [Isoform 3]: Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).

Research_area:Others

Applications:ELISA

Target_protein:LIMK1

Reactivity:Human

Assay type:Sandwich

Sensitivity:9.37ng/L

Detection range:20-4500ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Lim Domain Kinase 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P53667

MASS(Da):72,585

GeneID:3984

Synonyms:LIM domain kinase 1;LIMK 1;LIMK1;LIMK-1

Gene names:LIMK1

Standard solution:4800ng/L