Human Leukotriene E4 (LT-E4) ELISA Kit

SKU: AE34836HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: N/A

Abbreviation: LT-E4

Alternative Names: N/A

Application: ELISA

Range: 0.625-40 ng/mL

Sensitivity: 0.156 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate LT-E4 in samples. An antibody specific for LT-E4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLT-E4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LT-E4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LT-E4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: A biologically active principle of SRS-A that is formed from LEUKOTRIENE D4 via a peptidase reaction that removes the glycine residue. The biological actions of LTE4 are similar to LTC4 and LTD4.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).