Human KLK12(Kallikrein 12) ELISA Kit

SKU: ELK3389

Size:96T

Reactivity:Human

Alternative Names:KLK-L5; KLKL5; Kallikrein-Related Peptidase 12; Kallikrein-like protein 5

Assay Type:Competitive Inhibition

Sensitivity:6.83 ng/mL

Standard:1000 ng/mL

Detection range:15.63-1000 ng/mL

Sample type:Serum, plasma, other biological fluids

Assay length:2h

Research Area:Enzyme & Kinase;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK12 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q9UKR0