Human Isocitrate Dehydrogenase, Nadp Cytoplasmic, IDH1 ELISA Kit

SKU: E3097Hu

Size:96 Tests-1 Kit

Background:Isocitrate dehydrogenase 1 (NADP+), soluble is an enzyme that in humans is encoded by the IDH1 gene on chromosome 2. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which uses NAD+ as the electron acceptor and the other NADP+. Five isocitrate dehydrogenases have been reported: three NAD+-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP+-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP+-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2,4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Research_area:Signal Transduction

Applications:ELISA

Target_protein:IDH1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.11ng/ml

Detection range:0.2-60ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Isocitrate dehydrogenase [NADP] cytoplasmic

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:O75874

MASS(Da):46,659

GeneID:3417

Synonyms:Cytosolic NADP-isocitrate dehydrogenase;HEL-216;IDCD;IDH;IDH1;IDP;IDPC;Isocitrate dehydrogenase [NADP] cytoplasmic;NADP(+-specific ICDH;Oxalosuccinate decarboxylase;PICD

Gene names:IDH1

Standard solution:80ng/ml