Human IgG2(Immunoglobulin G2) ELISA Kit

SKU: ELK2143

Size:96T

Reactivity:Human

Alternative Names:IGHG2; Ig Gamma-2 Chain C Region; Immunoglobulin Heavy Constant Gamma 2; G2m Marker; Immunoglobulin Gm2

Assay Type:Sandwich

Sensitivity:0.51 µg/mL

Standard:100 µg/mL

Detection range:1.57-100 µg/mL

Sample type:Serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Infection immunity;Immune molecule;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IgG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IgG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgG2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P01859