Human Hepc 25(Hepcidin 25) ELISA Kit

SKU: ELK10037

Size:96T

Reactivity:Human

Alternative Names:Hamp; HEPC; Hepc20; Hepc25; HEPCIDIN; Hepcidin 20; Hepcidin 25; Hepcidin antimicrobial peptide; Hepcidin-20; Hepcidin25; HFE2; HFE2B; LEAP 1; LEAP-1; LEAP1; Liver expressed antimicrobial peptide; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor

Assay Type:Sandwich

Sensitivity:3.9 ng/mL

Standard:1000 ng/mL

Detection range:15.63-1000 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Metabolic pathway;Endocrinology;Hematology;Genetic science;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Hepc 25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Hepc 25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Hepc 25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Hepc 25 in the samples is then determined by comparing the OD of the samples to the standard curve.

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