Human GPNMB(Transmembrane glycoprotein NMB) Microsample ELISA Kit

SKU: ELK8760MS

Size:96T

Reactivity:Human

Alternative Names:Glycoprotein (transmembrane) nmb; Glycoprotein nmb; Glycoprotein nmb like protein; Gpnmb; GPNMB_HUMAN; HGFIN; NMB; Osteoactivin; Transmembrane glycoprotein; Transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB

Assay Type:Sandwich

Sensitivity:0.3 ng/mL

Standard:50 ng/mL

Detection range:0.79-50 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Cancer

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPNMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPNMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPNMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPNMB in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q14956