Human Flotillin-1 (FLOT1) ELISA Kit

SKU: AE58745HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: O75955

Abbreviation: FLOT1

Alternative Names: DADB-118P11.2; integral membrane component of caveolae

Application: ELISA

Range: 0.78-50 ng/mL

Sensitivity: 1.18 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate FLOT1 in samples. An antibody specific for FLOT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFLOT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FLOT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FLOT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Flot1 encodes a deduced 428-amino acid protein with a predicted molecular mass of 47 kD. It contains 2 hydrophobic domains and several potential phosphorylation sites. Flot1 shares 47% amino acid identity with both mouse and human FLOT2. Northern blot analysis of mouse tissues revealed expression in adipose tissue, heart, skeletal muscle, and lung. By Western blot analysis, Flot1 was also found as a 47-kD protein in caveolin-rich membrane domains isolated from human lung and from cultured mouse adipocytes. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase. Protein levels of PTOV1 and flotillin-1 oscillated during the cell cycle, with a peak in S phase.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).