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GeneBio Systems

Human F-Testo(Free Testosterone) ELISA Kit

Human F-Testo(Free Testosterone) ELISA Kit

ELK10969

£465.00 GBP
£465.00 GBP
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Size:96T

Reactivity:Human

Alternative Names:F-TESTO; Free Testosterone

Assay Type:Competitive Inhibition

Sensitivity:15.75 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:serum, plasma, other biological fluids

Assay length:2h

Research Area:Endocrinology;Reproductive science;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F-Testo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-Testo in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:

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