Human F-actin(Filamentous Actin) ELISA Kit

SKU: ELK0113

Size:96T

Reactivity:Human

Alternative Names:Fibrous Actin

Assay Type:Sandwich

Sensitivity:0.18 ng/mL

Standard:40 ng/mL

Detection range:0.63-40 ng/mL

Sample type:Serum, plasma, tissue homogenates

Assay length:3.5h

Research Area:Signal transduction;Developmental science;Bone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-actin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-actin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-actin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-actin in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: