Human DNA Topoisomerase 1, TOP1 ELISA Kit

SKU: E3605Hu

Size:96 Tests-1 Kit

Background:Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:TOP1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.12ng/ml

Detection range:0.2-70ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Dna Topoisomerase 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P11387

MASS(Da):90,726

GeneID:7150

Synonyms:DNA topoisomerase 1;DNA topoisomerase I;TOP 1;TOP1

Gene names:TOP1

Standard solution:80ng/ml