Human cytochrome P450, family 27, subfamily B, polypeptide 1,cyp27b1 ELISA KIT

SKU: E6847Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR) (PubMed:10518789, PubMed:9486994, PubMed:22862690, PubMed:10566658, PubMed:12050193). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway (PubMed:10518789, PubMed:22862690). Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2 (PubMed:10518789). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:22862690). {ECO:0000269|PubMed:10518789, ECO:0000269|PubMed:10566658, ECO:0000269|PubMed:12050193, ECO:0000269|PubMed:22862690, ECO:0000269|PubMed:9486994}.

Research_area:

Applications:ELISA

Target_protein:CYP27B1

Reactivity:Human

Assay type:Sandwich

Sensitivity:48.3pg/ml

Detection range:100-6400pg/ml

Sample type:Serum, plasma or other biological fluids

Full product name:cytochrome P450, family 27, subfamily B, polypeptide 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:O15528

MASS(Da):56504

GeneID:1594

Synonyms:CYP27B1 CYP1ALPHA CYP27B

Gene names:CYP27B1

Standard solution:12800pg/ml