Human Cytochrome P450 19A1,CYP19A1 ELISA KIT

SKU: E3077Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively (PubMed:27702664, PubMed:2848247). Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid (PubMed:20385561). Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen (PubMed:22773874). Also displays 2-hydroxylase activity toward estrone (PubMed:22773874). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:20385561, PubMed:22773874). {ECO:0000269|PubMed:20385561, ECO:0000269|PubMed:22773874, ECO:0000269|PubMed:27702664, ECO:0000269|PubMed:2848247}.

Research_area:

Applications:ELISA

Target_protein:CYP19A1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.1ng/ml

Detection range:0.19-12ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Cytochrome P450 19A1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P11511

MASS(Da):57883

GeneID:1588

Synonyms:CYP19A1 ARO1 CYAR CYP19

Gene names:CYP19A1

Standard solution:24ng/ml