Human Anti-TTN(Anti-Titin Antibody) ELISA Kit

SKU: ELK0760

Size:96T

Reactivity:Human

Alternative Names:CMD1G; CMH9; CMPD4; HMERF; LGMD2J; TMD; Cardiomyopathy,Dilated 1G(Autosomal Dominant)

Assay Type:Competitive Inhibition

Sensitivity:0.14 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:cell lysates, cell culture supernates and other biological fluids

Assay length:2.5h

Research Area:Developmental science;Bone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-TTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-TTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-TTN in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: