Human Acid Ceramidase, ASAH1 ELISA Kit

SKU: E3476Hu

Size:96 Tests-1 Kit

Background:Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH (PubMed: 10610716, PubMed: 7744740, PubMed: 15655246, PubMed: 11451951). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed: 10610716). Has a higher catalytic efficiency towards C12-ceramides versus other ceramides (PubMed: 7744740, PubMed: 15655246). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed: 12764132, PubMed: 12815059). For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used (PubMed: 12764132). Has also an N-acylethanolamine hydrolase activity (PubMed: 15655246). By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes (PubMed: 17713573). Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis (By similarity). By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis (PubMed: 22261821). [Isoform 2]: May directly regulate steroidogenesis by binding the nuclear receptor NR5A1 and negatively regulating its transcriptional activity.

Research_area:Others

Applications:ELISA

Target_protein:ASAH1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.28ng/ml

Detection range:0.5-180ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Acid Ceramidase

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q13510

MASS(Da):44,660

GeneID:427

Synonyms:AC;ACDase;Acid CDase;Acid ceramidase;Acid ceramidase subunit alpha;Acid ceramidase subunit beta;Acylsphingosine deacylase;ASAH 1;ASAH1;N-acylethanolamine hydrolase ASAH1;N-acylsphingosine amidohydrolase;PHP32;Putative 32 kDa heart protein;

Gene names:ASAH1

Standard solution:200ng/ml