Human 8-OHdG(DNA Damage) ELISA Kit

SKU: ELK10908

Size:96T

Reactivity:Human

Alternative Names:8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 8-Hydroxydeoxyguanosine

Assay Type:Competitive Inhibition

Sensitivity:0.59 ng/mL

Standard:100 ng/mL

Detection range:1.56-100 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

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