Human 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit

SKU: ELK0778

Size:96T

Reactivity:Human

Alternative Names:2,3DPG

Assay Type:Sandwich

Sensitivity:0.14 µmol/mL

Standard:20 µmol/mL

Detection range:0.32-20 µmol/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Metabolic pathway

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve.

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