GeneBio Systems

HSP70 ELISA kit (Human)

Name: HSP70 ELISA kit (Human)
Brand: GeneBio Systems
SKU: SEA873Hu
Price: 769.00 GBP
Availability: InStock

SKU:SEA873Hu

£769.00 GBP

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Quantity

Size: 96Tests

# of Times Cited in literature: 15

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: HSP70

Target Full Name: Heat Shock Protein 70

Alternative Names: DnaK; 70 Kilodalton Heat Shock Proteins

Target Species: Human

Uniprot: P0DMV8

Gene ID: 3303

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human HSP70 / Heat Shock Protein 70

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 3.12-200ng/mL

Sensitivity: 1.31ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 70 (HSP70) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 70 (HSP70) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 70 (HSP70). No significant cross-reactivity or interference between Heat Shock Protein 70 (HSP70) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 70 (HSP70). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 70 (HSP70). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 70 (HSP70), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 70 (HSP70) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Tumor immunity;Infection immunity;Developmental science;

References Citing This Product: Proteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathy

Neuroinflammation in autism spectrum disorders

Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder

Concentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del sue

Heat Shock Proteins,

L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients

Elevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migraine

Physiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid load

High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver.

SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death.

Concentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer