GFI1B Antibody - Cat. #: CSB-PA257884

SKU: CSB-PA257884

Size :100ul

Clone Number:

Aliases:GFI 1B antibody; gfi1b antibody; GFI1B protein antibody; GFI1B_HUMAN antibody; Growth factor independent 1B protein antibody; Growth factor independent 1B transcription repressor antibody; Growth factor independent protein 1B antibody; OTTHUMP00000022443 antibody; OTTHUMP00000022444 antibody; OTTHUMP000000235527 antibody; Potential regulator of CDKN1A antibody; Potential regulator of CDKN1A translocated in CML antibody; Translocated in CML antibody; Zinc finger protein Gfi-1b antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:Q5VTD9

Immunogen:Peptide sequence around aa.322~326(H-R-E-S-Q) derived from Human GFI1b.

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB; WB:1:500-1:1000

Background:

Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.

Osawa M., Yamaguchi T., Nakamura Y. Iwama A.Blood 100:2769-2777(2002)
Huang D.-Y., Kuo Y.-Y., Chang Z.-F. Nucleic Acids Res. 33:5331-5342(2005)
Vassen L., Fiolka K., Moeroey T. EMBO J. 25:2409-2419(2006)

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Conjugate:Non-conjugated

Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:GFI1B

Research Areas:Epigenetics and Nuclear Signaling;Cardiovascular