GCG ELISA kit (Mouse)

SKU: CEB266Mu

Size: 96Tests

# of Times Cited in literature: 16

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: GCG

Target Full Name: Glucagon

Alternative Names: GC; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone

Target Species: Mouse

Uniprot: P55095

Gene ID: 14526

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Mouse GCG / Glucagon

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 18.52-1,500pg/mL

Sensitivity: 7.47pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucagon (GCG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucagon (GCG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Glucagon (GCG). No significant cross-reactivity or interference between Glucagon (GCG) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Glucagon (GCG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glucagon (GCG) and unlabeled Glucagon (GCG) (Standards or samples) with the pre-coated antibody specific to Glucagon (GCG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glucagon (GCG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glucagon (GCG) in the sample.

Research Area: Endocrinology;

References Citing This Product: PER2 promotes glucose storage to liver glycogen during feeding and acute fasting by inducing Gys2 PTG and GL expression

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Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice

Effects of Acute Cold Stress on Liver O-GlcNAcylation and Glycometabolism in Mice

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Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose …

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