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Gene Bio Systems

GB-Script™ III 1 st Strand cDNA Synthesis Kit (+ gDNA wiper)

GB-Script™ III 1 st Strand cDNA Synthesis Kit (+ gDNA wiper)

SKU:R312-01

Regular price £236.00 GBP
Regular price Sale price £236.00 GBP
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This product delivers high efficiency of first strand cDNA synthesis. The 5× gDNA wiper Mix in the kit can quickly remove genomic DNA contamination at 42℃ for 2 min, which makes the results more reliable and simplifies qPCR primer design process without the need to design primers across introns. The kit contains two separated primers: the reverse transcription primers Oligo (dT) 20 VN and Random hexamers, allowing users to choose primers flexibly for subsequent experiments as needed. The kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize a highly uniform cDNA for qPCR quantification.


Components

R312-01 50rxn (20μl/rxn)

R312-02 100rxn (20μl/rxn)

RNasefreeddH2O

1 ml

1 ml

5× gDNAwiperMix

100 μl

200 μl

10x RTMix

100 μl

200 μl

GB-Script™ III EnzymeMix

100 μl

200 μl

Oligo (dT)20VN

50 μl

100 μl

RandomHexamers

50 μl

100 μl

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