GAL4 DNA Binding Domain rabbit pAb

SKU: ES20743

Size: 100μL

Source:Rabbit

Applications:WB

Reactivity:

Dilution:WB: 1:10000-50000

Immunogen:Recombinant Protein of GAL4 DNA Binding Domain

Storage_stability:-20°C/1 year

Clonality:Polyclonal

Isotype:IgG

Concentration:

Observed_band(KD):

Human_gene_id:

Human_swiss_prot_no:

Subcellular_location:

Other_name:YM3411

Background:The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation , this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique "two-hybrid" system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions.