EasyStep Human VEGF165(Vascular Endothelial Growth Factor 165) ELISA Kit

SKU: ELK027ES

Size:96T

Reactivity:Human

Alternative Names:VEGF165

Assay Type:Sandwich

Sensitivity:15 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Cytokine;Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGF165, and the Human VEGF165 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGF165. After TMB substrate solution is added, only those wells that contain Human VEGF165 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGF165 in the samples is then determined by comparing the OD of the samples to the standard curve.

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