EasyStep Human PDL1(Programmed Cell Death Protein 1 Ligand 1) ELISA Kit

SKU: ELK084ES

Size:96T

Reactivity:Human

Alternative Names:CD274; PDL1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1; PDCD1LG1; Programmed Cell Death Protein 1 Ligand 1

Assay Type:Sandwich

Sensitivity:1.23 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Autoimmunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDL1, and the Human PDL1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PDL1. After TMB substrate solution is added, only those wells that contain Human PDL1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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