EasyStep Human cTnI(Cardiac Troponin I) ELISA Kit

SKU: ELK057ES

Size:96T

Reactivity:Human

Alternative Names:TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Cardiac troponin I; Troponin I

Assay Type:Sandwich

Sensitivity:19.6 pg/mL

Standard:25 ng/mL

Detection range:0.39-25 ng/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Cardiovascular biology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human cTnI, and the Human cTnI standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human cTnI. After TMB substrate solution is added, only those wells that contain Human cTnI and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human cTnI in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P19429