Dog LTF(Lactoferrin) ELISA Kit

SKU: ELK10818

Size:96T

Reactivity:Dog

Alternative Names:HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

Assay Type:Sandwich

Sensitivity:18.3 ng/mL

Standard:5000 ng/mL

Detection range:78.13-5000 ng/mL

Sample type:serum, plasma, breast milk and other biological fluids

Assay length:3.5h

Research Area:Tumor immunity;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog LTF in the samples is then determined by comparing the OD of the samples to the standard curve.

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