Chicken T4(Thyroxine) ELISA Kit

SKU: ELK8957

Size:96T

Reactivity:Chicken

Alternative Names:3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

Assay Type:Competitive Inhibition

Sensitivity:1.49 ng/mL

Standard:300 ng/mL

Detection range:4.69-300 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Endocrinology;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken T4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: