Chicken GPX3(Glutathione Peroxidase 3) ELISA Kit

SKU: ELK0130

Size:96T

Reactivity:Chicken

Alternative Names:GSHPX3; GPx-P; GSHPx-P; GPXP; Plasma glutathione peroxidase; Extracellular glutathione peroxidase

Assay Type:Sandwich

Sensitivity:21.2 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:Serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken GPX3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GPX3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken GPX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GPX3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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