CETP ELISA kit (Human)

SKU: SEA814Hu

Size: 96Tests

# of Times Cited in literature: 5

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: CETP

Target Full Name: Cholesteryl Ester Transfer Protein

Alternative Names: LTP; Lipid Transfer Protein; Plasma Lipid Transfer Protein; Cholesterylester Transfer Protein

Target Species: Human

Uniprot: P11597

Gene ID: 1071

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human CETP / Cholesteryl Ester Transfer Protein

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 3.12-200ng/mL

Sensitivity: 1.29ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholesteryl Ester Transfer Protein (CETP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholesteryl Ester Transfer Protein (CETP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Cholesteryl Ester Transfer Protein (CETP). No significant cross-reactivity or interference between Cholesteryl Ester Transfer Protein (CETP) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholesteryl Ester Transfer Protein (CETP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholesteryl Ester Transfer Protein (CETP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholesteryl Ester Transfer Protein (CETP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholesteryl Ester Transfer Protein (CETP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Metabolic pathway;Hepatology;

References Citing This Product: 27-Hydroxycholesterol regulates cholesterol synthesis and transport in C6 glioma cells.

A baru almond-enriched diet reduces abdominal adiposity and improves HDL concentrations: A randomized, placebo-controlled trial

CETP genetic variant rs1800777 (allele A) is associated with abnormally low HDL-C levels and increased risk of AKI during sepsis

A baru almond└ enriched diet reduces abdominal adiposity and improves high-density lipoprotein concentrations: a randomized, placebo-controlled trial

Association of Serum Cholesterol Ester Transfer Protein Levels with Taq IB Polymorphism in Acute Coronary Syndrome