Cattle THP(Tamm-Horsfall Glycoprotein) ELISA Kit

SKU: ELK9723

Size:96T

Reactivity:Cattle

Alternative Names:THP; Uromucoid,Tamm-Horsfall Glycoprotein; Tamm-Horsfall urinary glycoprotein; Uromodulin; UMOD

Assay Type:Sandwich

Sensitivity:0.269 ng/mL

Standard:40 ng/mL

Detection range:0.63-40 ng/mL

Sample type:serum, plasma, urine and other biological fluids

Assay length:3.5h

Research Area:Kidney biomarker;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle THP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle THP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle THP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle THP in the samples is then determined by comparing the OD of the samples to the standard curve.

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