Caspase 8 Activity Detection Substrate for Flow Cytometry

SKU: E-CK-A488

Size:100Tests

Storage:This product can be stored at -20℃ for 1 year in the dark.

Shipping:Gel Packs

Exp date:12 months

Category ID_I:Apoptosis and Cell Health Detection

Category ID_II:Cell Apoptosis

Category ID_III:Caspase Assay Kits

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Kit Type:Apoptosis/Caspase

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Detection Instrument:Flow Cytometer;Fluorescence microscope

Tested Sample Types:Cell samples

Assay Time:30 min

Detection Type:Fluorometric method;Flow cytometry

Background:

Test Principle:The Caspase 8 Activity Detection Substrate for Flow Cytometry are a novel fluorogenic dye that couple the caspase 8 recognition sequence (IETD) to a high affinity DNA dye, which have cell membrane permeability and can penetrate the plasma membrane into the cytoplasm. The substrate itself has no fluorescence and has a charge exclusion effect with DNA. During cell apoptosis, caspase-8 cleaves the substrate and releases high affinity DNA dyes, which bind to DNA to produce strong fluorescence, thereby detecting caspase-8 activity and visualizing the morphological changes of the cell nucleus during apoptosis.

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