Caspase 6 Activity Assay Kit(Colorimetric Method)

SKU: E-CK-A386

Size:100Assays

Storage:This product can be stored at -20°C for 12 months with shading light.

Shipping:Gel Packs

Exp date:12 months

Category ID_I:Apoptosis and Cell Health Detection

Category ID_II:Cell Apoptosis

Category ID_III:Caspase Assay Kits

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Kit Type:Apoptosis/Caspase

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Result Type:Qualitative or quantitative

Detection Instrument:Spectrophotometer;Microplate reader

Tested Sample Types:Cell and tissue samples

Assay Time:4-6 hours

Detection Type:Colorimetric method

Background:

Test Principle:Caspase 6, also known as Mch-2, was originally discovered in human Jurkat cells. The precursor of caspase 6 can be cleaved by granzyme B to form an activated caspase 6 dimer, which is found to induce apoptosis. Caspase 6 can cleave PARP and keratin-18, and also cleave Lamin A, a key component protein on the nuclear envelope. Only caspase 6 can cleave Lamin A in the caspase family. This caspase 6 activity assay kit is based on caspase 6 that can catalyze the substrate Ac-VEID-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 6 can be calculated by determining the absorbance at 405 nm.

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