Caffeine ELISA Kit

SKU: ELK0683

Size:96T

Reactivity:General

Alternative Names:Caffeine

Assay Type:Competitive Inhibition

Sensitivity:0.25 ng/mL

Standard:30 ng/mL

Detection range:0.47-30 ng/mL

Sample type:Serum, plasma, other biological fluids

Assay length:2.5h

Research Area:Signal transduction;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Caffeine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Caffeine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caffeine in the samples is then determined by comparing the OD of the samples to the standard curve.

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