Bovine Poly(U)-binding-splicing factor PUF60,PUF60 ELISA KIT

SKU: E2244Bo

Size:96 Tests-1 Kit

Background:DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity). {ECO:0000250}.

Research_area:

Applications:ELISA

Target_protein:PUF60

Reactivity:Bovine

Assay type:Sandwich

Sensitivity:8.83mg/L

Detection range:15-3000mg/L

Sample type:Serum, plasma or other biological fluids

Full product name:Poly(U)-binding-splicing factor PUF60

Species:Bovine

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q2HJG2

MASS(Da):57087

GeneID:512824

Synonyms:PUF60

Gene names:PUF60

Standard solution:3200mg/L