Anti-XRCC1 Antibody Picoband®

SKU: PA1639

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Rat,Mouse,Human

Applications: IHC,ICC,WB

Application Details: Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: XRCC1

Specificity: No cross reactivity with other proteins.

Background: XRCC1 (X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC1, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Bhattacharyya, N., Banerjee, S. A novel role of XRCC1 in the functions of a DNA polymerase beta variant. Biochemistry 40: 9005-9013, 2001. 2. Brookman, K. W., Tebbs, R. S., Allen, S. A., Tucker, J. D., Swiger, R. R., Lamerdin, J. E., Carrano, A. V., Thompson, L. H. Isolation and characterization of mouse Xrcc1, a DNA repair gene affecting ligation. Genomics 22: 180-188, 1994.

Uniprot ID: P18887

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 90 kDa

Calculated Molecular Weight: 69477 MW

Gene ID: 7515

Protein Name: DNA repair protein XRCC1

Gene Full Name: X-ray repair cross complementing 1

Synonyms: DNA repair protein XRCC1; RCC; XRCC1