Anti-WDR77 Antibody Picoband®

SKU: A04894-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: WDR77

Specificity:

Background: Methylosome protein 50 is a protein that in humans is encoded by the WDR77 gene. The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human WDR77 recombinant protein (Position: A13-A295).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Friesen, W. J., Wyce, A., Paushkin, S., Abel, L., Rappsilbers, J., Mann, M., Dreyfuss, G. A novel WD repeat protein component of the methylosome binds Sm proteins. J. Biol. Chem. 277: 8243-8247, 2002. 2. Furuno, K., Masatsugu, T., Sonoda, M., Sasazuki, T., Yamamoto, K. Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro. Biochem. Biophys. Res. Commun. 345: 1051-1058, 2006. 3. Gross, M. B. Personal Communication. Baltimore, Md. 3/2/2018.

Uniprot ID: Q9BQA1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 42 kDa

Calculated Molecular Weight: 56903 MW

Gene ID: 79084

Protein Name: Methylosome protein WDR77

Gene Full Name: WD repeat domain 77

Synonyms: Androgen receptor cofactor p44; HKMT1069; MEP 50; MEP50; Methylosome protein 50; Nbla10071; p44; p44/Mep50; RP11 552M11.3; WD repeat domain 77; WDR77