Anti-TRPM8 Antibody Picoband®

SKU: PB9837

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: TRPM8

Specificity: No cross reactivity with other proteins.

Background: Transient receptor potential cation channel subfamily M member 8 (TRPM8), also known as the cold and menthol receptor 1 (CMR1), is a protein that in humans is encoded by the TRPM8 gene. TRPM8 is an ion channel, upon activation it allows the entry of Na+ (sodium) and Ca2+ (calcium) ions to the cell that leads to depolarization and the generation of an action potential. The signal is conducted from primary afferents (type C- and A-delta) eventually leading to the sensation of cold and cold pain. The TRPM8 protein is expressed in sensory neurons, and it is activated by cold temperatures and cooling agents, such as menthol and icilin whereas WS-12 and CPS-369 are the most selective agonist of TRPM8.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TRPM8, different from the related mouse sequence by four amino acids, and from the related rat sequence by two amino acids.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Andersen HH, Møller G, Winter R, Eskelund P, Arendt-Nielsen L (Aug 2013). A review of topical high-concentration L-menthol as a translational model of cold allodynia and hyperalgesia. European Journal of Pain 18 (3): 315–325. 2. Sherkheli M.A.; et al. (2007). Selective TRPM8 agonists: a novel group of neurophathic analgesics. FEBS Journal274 (s1): 232. 3. Sherkheli M.A., G G, Ak VE, Jf D, H H (October 2008). Menthol derivative WS-12 selectively activates transient receptor potential melastatin-8 (TRPM8) ion channels. Pak J Pharm Sci 21 (4): 370–8.

Uniprot ID: Q7Z2W7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 127 kDa

Calculated Molecular Weight: 127685 MW

Gene ID: 79054

Protein Name: Transient receptor potential cation channel subfamily M member 8

Gene Full Name: transient receptor potential cation channel subfamily M member 8

Synonyms: LTrpC 6; LTRPC6; Trp p8; TRPM8; TRPP8