Anti-TRIM3 Antibody Picoband®

SKU: A07081-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TRIM3

Specificity:

Background: Tripartite motif-containing protein 3 is a protein that in humans is encoded by the TRIM3 gene. The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified.

Immunogen: E.coli-derived human TRIM3 recombinant protein (Position: Q77-R433).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cheung, C. C., Yang, C., Berger, T., Zaugg, K., Reilly, P., Elia, A. J., Wakeham, A., You-Ten, A., Chang, N., Li, L., Wan, Q., Mak, T. W. Identification of BERP (brain-expressed RING finger protein) as a p53 target gene that modulates seizure susceptibility through interacting with GABA(A) receptors. Proc. Nat. Acad. Sci. 107: 11883-11888, 2010. 2. El-Husseini, A. E., Kwasnicka, D., Yamada, T., Hirohashi, S., Vincent, S. R. BERP, a novel ring finger protein, binds to alpha-actinin-4. Biochem. Biophys. Res. Commun. 267: 906-911, 2000. 3. El-Husseini, A. E., Vincent, S. R. Cloning and characterization of a novel RING finger protein that interacts with class V myosins. J. Biol. Chem. 274: 19771-19777, 1999.

Uniprot ID: O75382

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 81 kDa

Calculated Molecular Weight:

Gene ID: 10612

Protein Name: Tripartite motif-containing protein 3

Gene Full Name: tripartite motif containing 3

Synonyms: BERP; FLJ16135; HAC1; RING finger protein 22; RING finger protein 97; RNF22; RNF97; TRIM3; tripartite motif containing 3