Anti-TMEM16A/ANO1 Antibody Picoband®

SKU: PA2290

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Gene Name: ANO1

Specificity: No cross reactivity with other proteins.

Background: Anoctamin-1 (ANO1), also known as oral cancer overexpressed 2 (ORAOV2) or tumor-amplified and overexpressed sequence 2 (TMEM16A), is a protein that in humans is encoded by the ANO1 gene. This gene belongs to a family of membrane proteins containing 8 transmembrane segments, and it is mapped to 11q13.3. ANO1 is a candidate calcium-activated chloride channel that mediates receptor-activated chloride currents in diverse physiologic processes, and it is though to be responsible for a voltage-sensitive calcium-activated chloride current. Its overexpression was reported in esophageal squamous cell carcinoma and breast cancer progression Crofelemer, an antidiarrhoeal, inhibits this channel. ANO1 has eight transmembrane domains, its pore is large and non-selective, allowing other negatively charged species to permeate.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM16A, different from the related mouse and rat sequences by one amino acid.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Caputo A, Caci E, Ferrera L, Pedemonte N, Barsanti C, Sondo E, Pfeffer U, Ravazzolo R, Zegarra-Moran O, Galietta LJ (October 2008). TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity. Science 322 (5901): 590–4. 2. Katoh M, Katoh M (June 2003). FLJ10261 gene, located within the CCND1-EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products. Int. J. Oncol. 22 (6): 1375–81. 3. Yang, Y. D., Cho, H., Koo, J. Y., Tak, M. H., Cho, Y., Shim, W.-S., Park, S. P., Lee, J., Lee, B., Kim, B.-M., Raouf, R., Shin, Y. K., Oh, U. TMEM16A confers receptor-activated calcium-dependent chloride conductance. Nature 455: 1210-1215, 2008.

Uniprot ID: Q5XXA6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 114 kDa

Calculated Molecular Weight: 114078 MW

Gene ID: 55107

Protein Name: Anoctamin-1

Gene Full Name: anoctamin 1

Synonyms: ANO1; Anoctamin 1; DOG1; ORAOV2; TAOS2; TMEM16A; TMEM16A/DOG1; Transmembrane protein 16A