Anti-TIP49A/RUVBL1 Antibody Picoband®

SKU: A02049-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: WB,IHC,ICC,IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RUVBL1

Specificity:

Background: RuvB-like 1 (E. coli), also known as RUVBL1 and TIP49, is a human gene. This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.

Immunogen: E.coli-derived human TIP49A/RUVBL1 recombinant protein (Position: Q13-D23).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bauer, A., Huber, O., Kemler, R. Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein. Proc. Nat. Acad. Sci. 95: 14787-14792, 1998. 2. Doyon, Y., Selleck, W., Lane, W. S., Tan, S., Cote, J. Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans. Molec. Cell. Biol. 24: 1884-1896, 2004. 3. Fazzio, T. G., Huff, J. T., Panning, B. An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity. Cell 134: 162-174, 2008.

Uniprot ID: Q9Y265

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 54 kDa

Calculated Molecular Weight: 37492 MW

Gene ID: 8607

Protein Name: RuvB-like 1

Gene Full Name: RuvB like AAA ATPase 1

Synonyms: 49 kDa TBP interacting protein; ECP 54; ECP54; INO80 complex subunit H; INO80H; Nuclear matrix protein 238; PONTIN; Pontin 52; Pontin52; RuvB like 1; RuvB like 1 (E. coli); RUVBL1; RVB1; TAP54 alpha; TIH1; TIP49; TIP49A