Anti-Thymidylate Synthase/TYMS Antibody Picoband®

SKU: A04320-4

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Monkey,Rat

Applications: ELISA,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TYMS

Specificity:

Background: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy.

Immunogen: E.coli-derived human Thymidylate Synthase/TYMS recombinant protein (Position: M1-V313).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chu, J., Dolnick, B. J. Natural antisense (rTS-alpha) RNA induces site-specific cleavage of thymidylate synthase mRNA. Biochim. Biophys. Acta 1587: 183-193, 2002. 2. Hori, T., Ayusawa, D., Shimizu, K., Koyama, H., Seno, T. Assignment of human gene encoding thymidylate synthase to chromosome 18 using interspecific cell hybrids between thymidylate synthase-negative mouse mutant cells and human diploid fibroblasts. Somat. Cell Molec. Genet. 11: 277-283, 1985. 3. Hori, T., Ayusawa, D., Shimizu, K., Koyama, H., Seno, T. Assignment of the human TMS gene, encoding thymidylate synthase, to chromosome 18. (Abstract) Cytogenet. Cell Genet. 40: 654-655, 1985.

Uniprot ID: P04818

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 36 kDa

Calculated Molecular Weight:

Gene ID: 7298

Protein Name: Thymidylate synthase

Gene Full Name: thymidylate synthetase

Synonyms: HsT422; Thymidylate synthase; thymidylate synthetase; TMS; TS; TSase; TYMS