Anti-Thymidine Kinase 2/TK2 Antibody Picoband®

SKU: A03321-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TK2

Specificity:

Background: Thymidine kinase 2, mitochondrial is a protein that in humans is encoded by the TK2 gene. This gene encodes a deoxyribonucleoside kinase that specifically phosphorylates thymidine, deoxycytidine, and deoxyuridine. The encoded enzyme localizes to the mitochondria and is required for mitochondrial DNA synthesis. Mutations in this gene are associated with a myopathic form of mitochondrial DNA depletion syndrome. Alternate splicing results in multiple transcript variants encoding distinct isoforms, some of which lack transit peptide, so are not localized to mitochondria.

Immunogen: E.coli-derived human Thymidine Kinase 2/TK2 recombinant protein (Position: V34-P265).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Akman, H. O., Dorado, B., Lopez, L. C., Garcia-Cazorla, A., Vila, M. R., Tanabe, L. M., Dauer, W. T., Bonilla, E., Tanji, K., Hirano, M. Thymidine kinase 2 (H126N) knockin mice show the essential role of balanced deoxynucleotide pools for mitochondrial DNA maintenance. Hum. Molec. Genet. 17: 2433-2440, 2008. 2. Bartesaghi, S., Betts-Henderson, J., Cain, K., Dinsdale, D., Zhou, X., Karlsson, A., Salomoni, P., Nicotera, P. Loss of thymidine kinase 2 alters neuronal bioenergetics and leads to neurodegeneration. Hum. Molec. Genet. 19: 1669-1677, 2010. 3. Behin, A., Jardel, C., Claeys, K. G., Fagart, J., Louha, M., Romero, N. B., Laforet, P., Eymard, B., Lombes, A. Adult cases of mitochondrial DNA depletion due to TK2 defect: an expanding spectrum. Neurology 78: 644-648, 2012.

Uniprot ID: O00142

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 25 kDa

Calculated Molecular Weight:

Gene ID: 7084

Protein Name: Thymidine kinase 2, mitochondrial

Gene Full Name: thymidine kinase 2

Synonyms: TK2; Thymidine kinase 2; mitochondrial; Mt-TK; 2'-deoxyuridine kinase TK2; Deoxycytidine kinase TK2