Anti-TGIF/TGIF1 Antibody Picoband®

SKU: A04122-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TGIF1

Specificity:

Background: Homeobox protein TGIF1 is a protein that, in humans, is encoded by the TGIF1 gene. The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described.

Immunogen: E.coli-derived human TGIF/TGIF1 recombinant protein (Position: D26-A401).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aguilella, C., Dubourg, C., Attia-Sobol, J., Vigneron, J., Blayau, M., Pasquier, L., Lazaro, L., Odent, S., David, V. Molecular screening of the TGIF gene in holoprosencephaly: identification of two novel mutations. Hum. Genet. 112: 131-134, 2003. 2. Bertolino, E., Reimund, B., Wildt-Perinic, D., Clerc, R. G. A novel homeobox protein which recognizes a TGT core and functionally interferes with a retinoid-responsive motif. J. Biol. Chem. 270: 31178-31188, 1995. 3. Bertolino, E., Wildt, S., Richards, G., Clerc, R. G. Expression of a novel murine homeobox gene in the developing cerebellar external granular layer during its proliferation. Dev. Dyn. 205: 410-420, 1996.

Uniprot ID: Q15583

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 43 kDa

Calculated Molecular Weight: 211344 MW

Gene ID: 7050

Protein Name: Homeobox protein TGIF1

Gene Full Name: TGFB induced factor homeobox 1

Synonyms: TGIF1; TGIF; Homeobox protein TGIF1; 5'-TG-3'-interacting factor 1