Anti-SYNJ2 Antibody Picoband®

SKU: A08206-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SYNJ2

Specificity:

Background: Synaptojanin 2 is a protein that in humans is encoded by the SYNJ2 gene. The gene is a member of the inositol-polyphosphate 5-phosphatase family. The encoded protein interacts with the ras-related C3 botulinum toxin substrate 1, which causes translocation of the encoded protein to the plasma membrane where it inhibits clathrin-mediated endocytosis. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human SYNJ2 recombinant protein (Position: D18-K1495). Human SYNJ2 shares 83.8% amino acid (aa) sequence identity with rat SYNJ2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chuang, Y., Tran, N. L., Rusk, N., Nakada, M., Berens, M. E., Symons, M. Role of synaptojanin 2 in glioma cell migration and invasion. Cancer Res. 64: 8271-8275, 2004. 2. Malecz, N., McCabe, P. C., Spaargaren, C., Qiu, R.-G., Chuang, Y., Symons, M. Synaptojanin 2, a novel Rac1 effector that regulates clathrin-mediated endocytosis. Curr. Biol. 10: 1383-1386, 2000. 3. Nagase, T., Ishikawa, K., Nakajima, D., Ohira, M., Seki, N., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 4: 141-150, 1997.

Uniprot ID: O15056

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 200 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 8871

Protein Name: Synaptojanin-2

Gene Full Name: synaptojanin 2

Synonyms: INPP5H, KIAA0348, synaptojanin 2, SYNJ2