Anti-SWAP70 Antibody Picoband®

SKU: A06972-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.5-1 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SWAP70

Specificity:

Background: SWAP70 (Switch-associated protein 70) is a 67-70 kDa intracellular protein that bears limited resemblance to members of the Dbl-family of GEFs for small Rho GTPases. It is expressed in mast cells, dendritic cells and B cells. In mast cells, it promotes the release of anaphylactic mediators; in dendritic cells, it regulates the appearance of MHC II; and in B cells, it forms a nuclear complex that initiates immunoglobulin class switching. Human SWAP70 is 585 amino acids (aa) in length. It contains one plextrin homology (PH) domain (aa 213-304) plus a coiled-coil region (aa 316-539). There are three potential splice variants. One contains an alternative start site at Met70, another shows a deletion of aa 81-138, and a third possesses a 38 aa substitution for aa 453-585. Over aa 386-585, human SWAP70 shares 96% aa identity with mouse SWAP70.

Immunogen: E.coli-derived human SWAP70 recombinant protein (Position: H161-K292).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Borggrefe, T., Masat, L., Wabl, M., Riwar, B., Cattoretti, G., Jessberger, R. Cellular, intracellular, and developmental expression patterns of murine SWAP-70. Europ. J. Immun. 29: 1812-1822, 1999. 2. Borggrefe, T., Wabl, M., Akhmedov, A. T., Jessberger, R. A B-cell-specific DNA recombination complex. J. Biol. Chem. 273: 17025-17035, 1998. 3. Masat, L., Caldwell, J., Armstrong, R., Khoshnevisan, H., Jessberger, R., Herndier, B., Wabl, M., Ferrick, D. Association of SWAP-70 with the B cell antigen receptor complex. Proc. Nat. Acad. Sci. 97: 2180-2184, 2000.

Uniprot ID: Q9UH65

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 69 kDa

Calculated Molecular Weight: 87148 MW

Gene ID: 23075

Protein Name: Switch-associated protein 70

Gene Full Name: switching B cell complex subunit SWAP70

Synonyms: SWAP70; KIAA0640; HSPC321; Switch-associated protein 70; SWAP-70