Anti-SUN5 Antibody Picoband®

SKU: A10237-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: SUN5

Specificity:

Background: The protein encoded by this gene appears to play a role in the meiotic stage of spermatogenesis. The encoded protein localizes to the junction between the sperm head and body and may be involved in nuclear envelope reconstitution and nuclear migration. Mutations in this gene have been implicated in acephalic spermatozoa syndrome.

Immunogen: E.coli-derived human SUN5 recombinant protein (Position: E16-K341). Human SUN5 shares 79.1% amino acid (aa) sequence identity with mouse SUN5.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Razafsky, D., Hodzic, D. Bringing KASH under the SUN: the many faces of nucleo-cytoskeletal connections. J. Cell Biol. 186: 461-472, 2009. 2. Xing, X.-W., Li, L.-Y., Fu, J.-J., Zhu, W.-B., Liu, G., Liu, S.-F., Lu, G.-X. Cloning of cDNA of TSARG4, a human spermatogenesis related gene. Acta Biochim. Biophys. Sinica 35: 283-288, 2003. 3. Yassine, S., Escoffier, J., Abi Nahed, R., Pierre, V., Karaouzene, T., Ray, P. F., Arnoult, C. Dynamics of Sun5 localization during spermatogenesis in wild type and Dpy19l2 knock-out mice indicates that Sun5 is not involved in acrosome attachment to the nuclear envelope. PLoS One 10: e0118698, 2015. Note: Electronic Article. Erratum: PLoS One 10: e0125452, 2015.

Uniprot ID: Q8TC36

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight: 43 kDa

Gene ID: 140732

Protein Name: SUN domain-containing protein 5

Gene Full Name: Sad1 and UNC84 domain containing 5

Synonyms: SPAG4L; SUN5; TSARG4