Anti-SPTBN2 Antibody Picoband®

SKU: A05492

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SPTBN2

Specificity:

Background: Spectrin beta chain, brain 2 is a protein that in humans is encoded by the SPTBN2 gene. Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements.

Immunogen: E.coli-derived human SPTBN2 recombinant protein (Position: A594-K1909).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Al-Muhaizea, M. A., AlMutairi, F., Almass, R., AlHarthi, S., Aldosary, M. S., Alsagob, M., AlOdaib, A., Colak, D., Kaya, N. A novel homozygous mutation in SPTBN2 leads to spinocerebellar ataxia in a consanguineous family: report of a new infantile-onset case and brief review of the literature. Cerebellum 17: 276-285, 2018. 2. Clarkson, Y. L., Gillespie, T., Perkins, E. M., Lyndon, A. R., Jackson, M. Beta-III spectrin mutation L253P associated with spinocerebellar ataxia type 5 interferes with binding to Arp1 and protein trafficking from the Golgi. Hum. Molec. Genet. 19: 3634-3641, 2010. 3. Clarkson, Y. L., Perkins, E. M., Cairncross, C. J., Lyndon, A. R., Skehel, P. A., Jackson, M. Beta-III spectrin underpins ankyrin R function in Purkinje cell dendritic trees: protein complex critical for sodium channel activity is impaired by SCA5-associated mutations. Hum. Molec. Genet. 23: 3875-3882, 2014.

Uniprot ID: O15020

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 271 kDa

Calculated Molecular Weight: 48608 MW

Gene ID: 6712

Protein Name: Spectrin beta chain, non-erythrocytic 2

Gene Full Name: spectrin beta, non-erythrocytic 2

Synonyms: Beta III spectrin; SCA5; Spectrin beta chain; brain 2; Spectrin beta-3; SPTBN2