Anti-SPHK2 Antibody Picoband®

SKU: A01382-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml

Gene Name: SPHK2

Specificity: No cross reactivity with other proteins.

Background: Sphingosine kinase 2 is a protein that in humans is encoded by the SPHK2 gene. This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen: E. coli-derived human SPHK2 recombinant protein (Position: F215-M269).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Hait NC, Sarkar S, Le Stunff H, Mikami A, Maceyka M, Milstien S, Spiegel S (August 2005). Role of sphingosine kinase 2 in cell migration toward epidermal growth factor. The Journal of Biological Chemistry. 280 (33): 29462–9. 2. Liu H, Sugiura M, Nava VE, Edsall LC, Kono K, Poulton S, Milstien S, Kohama T, Spiegel S (June 2000). Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform. The Journal of Biological Chemistry. 275 (26): 19513–20.

Uniprot ID: Q9NRA0

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 69 kDa

Calculated Molecular Weight:

Gene ID: 56848

Protein Name: Sphingosine kinase 2

Gene Full Name: sphingosine kinase 2

Synonyms: SK 2; sphingosine kinase 2; SPHK2; SPK 2