Anti-SOS1 Antibody Picoband®

SKU: A00837-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SOS1

Specificity:

Background: Son of sevenless homolog 1 is a protein that in humans is encoded by the SOS1 gene. This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4.

Immunogen: E.coli-derived human SOS1 recombinant protein (Position: H177-A1288). Human SOS1 shares 96.5% amino acid (aa) sequence identity with mouse SOS1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bowtell, D., Fu, P., Simon, M. A., Senior, P. Identification of murine homologues of the Drosophila Son of sevenless gene: potential activators of ras. Proc. Nat. Acad. Sci. 89: 6511-6515, 1992. 2. Chardin, P., Camonis, J. H., Gale, N. W., Van Aelst, L., Schlessinger, J., Wigler, M. H., Bar-Sagi, D. Human Sos1: a guanine nucleotide exchange factor for Ras that binds to GRB2. Science 260: 1338-1343, 1993. 3. Ferrero, G. B., Baldassarre, G., Delmonaco, A. G., Biamino, E., Banaudi, E., Carta, C., Rossi, C., Silengo, M. C. Clinical and molecular characterization of 40 patients with Noonan syndrome. Europ. J. Med. Genet. 51: 566-572, 2008.

Uniprot ID: Q07889

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 170 kDa

Calculated Molecular Weight: 152 kDa

Gene ID: 6654

Protein Name: Son of sevenless homolog 1

Gene Full Name: SOS Ras/Rac guanine nucleotide exchange factor 1

Synonyms: GF1; GGF1; GINGF; NS4; Son of sevenless homolog 1; SOS 1; SOS1